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G005482

LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec® CHIROBIOTIC® R

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Astec CHIROBIOTIC R, 15 cm x 2.1 mm I.D., 5 μm particles (13019AST)
mobile phase: [A] 33.3 mM ammonium acetate; [B] acetonitrile; (15:85, A:B)
flow rate: 0.7 mL/min
column temp.: 4 °C
detector: (-)ESI
injection: 20 μL
sample: Urine extracts in 5% (v/v) acetic acid, 10% (v/v) H2O and 85% (v/v) acetonitrile

suitability

application for HPLC

application(s)

clinical

Analysis Note

Chromatogram supplied courtesy of H. Henry and associates at the Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH-1011, Switzerland. View the related full-length contributed article in Reporter 30.1.

Other Notes

Reference: Biomed. Chromatogr. 2011 "Sensitive determination of D-lactic acid and L-lactic acid in urine by high-performance liquid chromatography–tandem mass spectrometry" H. Henry, N. Marmy Conus, P. Steenhout, A. Béguin and O. Boulat, Published online in Wiley Online Library, wileyonlinelibrary.com/journal/bmc.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    L-(+)-Lactic acid, analytical standard
    D-(−)-Lactic acid, ≥90%

analytical column

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