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G003710

HPLC Analysis of Nucleosides on Ascentis® Phenyl

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Phenyl, 15 cm x 2.1 mm I.D., 5 μm particles (581613-U)
mobile phase: 10 mM ammonium formate (pH 3.0 with formic acid)
flow rate: 0.2 mL/min
column temp.: 35 °C
detector: UV, 270 nm; 750 psi back pressure regulator on outlet of flow cell
injection: 1 μL
sample: as indicated, in mobile phase

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Cytidine, ≥99.0% (HPLC)
    2′-Deoxycytidine hydrate, ≥99% (HPLC)
    Guanosine, ≥98%
    Uridine, ≥99%
    Adenosine, ≥99%
    3′-Deoxyguanosine .1 mg/mL
    5′-Deoxyadenosine, methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase substrate .1 mg/mL
    Thymidine, ≥99.0% (HPLC) .1 mg/mL
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