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817500

TSKgel® Octadecyl-2PW HPLC Column

phase C18 (octadecyl), L × I.D. 15 cm × 4.6 mm, 5 μm particle size

Synonym(s):

TSKgel® Reversed Phase HPLC Column (Polymer-based)

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About This Item

UNSPSC Code:
41115711
eCl@ss:
32110501

material

stainless steel hardware

product line

TSKgel®

manufacturer/tradename

Tosoh 17500

extent of labeling

1 meq/mL ligand density (estimate)

parameter

0-100% organic solvent
1.2 mL/min flow rate
120 bar max. pressure
4-50 °C temperature

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

matrix

polymer particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

125 Å

operating pH

2-12

separation technique

reversed phase

General description

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-2PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 125Å pores allow for anaylsis of peptides up to 8,000 Da. Columns packed with larger particle sizes are available for semi-preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
  • Octadecyl-2PW particles have 125Å pores
  • Octadecyl-4PW particles have 500Å pores
  • Phenyl-5PW RP particles have 1000Å pores
  • Octadecyl-NPR particles are nonporous

Physical form

Shipped in 55% acetonitrile - 45% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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