50632-U
Ascentis® Express 90 Å F5 (5 μm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonym(s):
Core-shell (SPP) Fused Core PFP HPLC column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
product name
Ascentis® Express F5, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L43
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
60 °C temp. range
600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
90 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
5 μm
pore size
90 Å pore size
operating pH
2-8
application(s)
food and beverages
separation technique
reversed phase
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General description
Ascentis® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Application
- Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employs an Ascentis® Express F5, 5 µm HPLC column for the precise determination of lumacaftor amidst ivacaftor and identifies novel degradation products, showcasing the column′s efficacy in complex mixture analysis (Özcan et al., 2023).
- Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: Utilizing the Ascentis® Express F5, 5 µm HPLC column, this research developed stability-indicating methods for tasimelteon, highlighting the column′s robustness in pharmaceutical analysis (Özcan et al., 2020).
- Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review explores the advancements in HPLC columns, including the Ascentis® Express F5, emphasizing its role in achieving ultra-fast separations in analytical chemistry (Ali et al., 2012).
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
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required but not provided
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Storage Class Code
11 - Combustible Solids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Iltaf Shah et al.
The Journal of steroid biochemistry and molecular biology, 180, 118-128 (2018-02-13)
Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 120, 112-119 (2016-01-01)
A new high-performance liquid chromatography method using fused-core column for fast separation of resveratrol and polydatin has been developed and used for quality control of nutraceuticals with resveratrol and polydatin content. Retention characteristics (log k) were studied under different conditions
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