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Z374903

Sigma-Aldrich

PCR multiwell plates

96 well plate for PCR

Synonym(s):

96 mulitwell PCR plate, 96 well PCR microplate, 96 well PCR plate, 96 well microtiter plate

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About This Item

UNSPSC Code:
41106300
NACRES:
NB.22

material

colorless polypropylene
colorless wells

sterility

non-sterile

feature

plate format: 96 well standard

packaging

case of 25 ea

manufacturer/tradename

Sorenson 2633

technique(s)

PCR: suitable

size

96 well

well maximum volume

300 μm

working volume

200 μL

suitability

suitable for (PCR, RT-PCR or DNA purification applications)

application(s)

agriculture

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General description

A rigid top plate (included) minimizes plate distortion, assures a dependable fit with the PCR thermal cycler, and allows for a leak-proof seal with micro-mats or cap strips. Each well has a maximum capacity of 200 μL.

Features and Benefits

  • Virgin polypropylene
  • Fully autoclavable
  • Certified DNase- and RNase-free
  • Wells have thin walls for rapid temperature equilibration and reduced cycle time

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Reverse Transcription Protocol (One-step Probe Detection)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.

qPCR Gene Expression Using Probe Detection

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

qPCR Using a Single Detection Probe Protocol

A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt® ReadyMix™.

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