Z374903
PCR multiwell plates
96 well plate for PCR
Synonym(s):
96 mulitwell PCR plate, 96 well PCR microplate, 96 well PCR plate, 96 well microtiter plate
About This Item
Recommended Products
material
colorless polypropylene
colorless wells
sterility
non-sterile
feature
plate format: 96 well standard
packaging
case of 25 ea
manufacturer/tradename
Sorenson 2633
technique(s)
PCR: suitable
size
96 well
well maximum volume
300 μm
working volume
200 μL
suitability
suitable for (PCR, RT-PCR or DNA purification applications)
application(s)
agriculture
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General description
Features and Benefits
- Virgin polypropylene
- Fully autoclavable
- Certified DNase- and RNase-free
- Wells have thin walls for rapid temperature equilibration and reduced cycle time
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecting group is removed during a heat activation step.
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt® ReadyMix™.
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