T1819
Trizma® hydrochloride solution
pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture
Synonym(s):
Tris hydrochloride solution
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.25
Recommended Products
grade
BioPerformance Certified
for molecular biology
Quality Level
form
liquid
concentration
1 M
technique(s)
cell culture | mammalian: suitable
impurities
DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)
pH
7.0
useful pH range
7.0-9.0
absorption
≤0.05 at 290 at 40%
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General description
Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.
Application
Trizma® hydrochloride solution has been used:
- as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii
- in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies
- for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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