S2519

Anti-Soy Protein antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

• MDL number: MFCD00162862
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: fractionated antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: soy
• technique(s): dot blot: 1:500; indirect ELISA: 1:5,000
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Some cross-reaction is observed with extracts from wheat flour and ovalbumin; no cross-reaction is observed with extracts from maize, rice, potato flour, casein, or meat.

Soy protein is obtained from soybeans. This legume is free of cholesterol and is low in saturated fat. It is mainly used as a replacement for animal proteins in an individual′s diet. This vegetable food has all eight essential amino acids. Soy protein can be used as meat extenders or analogues.

Immunogen

renatured soy protein extracted from soy acetone powder with hot urea.

Application

Anti-Soy Protein antibody produced in rabbit has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• supershift analysis
• fluorescent imaging
• immunostaining to probe polyvinylene diflouride (PVDF)-bound proteins for cross-reactivity

Anti-Soy Protein antibody produced in rabbit was used for soy quantification in natural rubber latex glove extracts by ELISA at 1:2000 dilution. It was used as a control in supershift analysis of growth-hormone-induced protein binding to lactogenic hormone-responsive region.

Biochem/physiol Actions

Soy protein is a rich source of isoflavones that act as estrogen analogs. They exert both estrogenic and anti-estrogenic effects depending on the tissue in which they act. Isoflavones effect the lipid and bone metabolism and may act as a potential alternative to estrogen in post-menopausal women.

Target description

Soy protein in a variety of forms is the basis of many low cholesterol foods and may also be incorporated in animal meat products.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable