P4439
Phosphatase, Alkaline from porcine kidney
lyophilized powder, ≥100 DEA units/mg protein
Synonym(s):
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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About This Item
Recommended Products
form
lyophilized powder
specific activity
≥100 DEA units/mg protein
composition
Protein, ≥50%
storage temp.
−20°C
Related Categories
Application
Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.
Packaging
Package size based on DEA units
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.
Physical form
Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0
Analysis Note
Protein determined by biuret.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
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