P3430

Monoclonal Anti-Phosphoserine antibody produced in mouse

clone PSR-45, ascites fluid

• Synonym(s): Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: PSR-45, monoclonal
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:4,000; western blot: 1:500-1:1,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Specificity

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Immunogen

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Application

Anti-Phosphoserine antibody may be used for indirect ELISA at a working dilution of 1:4000. For immunoblotting using rat brain cortex extracts, a working dilution of 1:500-1:1100 may be used. The antibody was used for immunoblotting to detect proteins phosphorylated at serine in stem extracts of Arabidopsis thalliana at a working dilution of 1:250.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)

Monoclonal Anti-Phosphoserine antibody produced in mouse has been used in western blotting.

Biochem/physiol Actions

Phosphoserine (PS) can be used as a template to detect cancer antigen 125 (CA 125).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable