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MAK004

Sigma-Aldrich

Glutamate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

neurological disorders; cancer

storage temp.

−20°C

Related Categories

General description

Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter. It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease. Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.

Application

Glutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model. It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.

Suitability

Suitable to determine glutamate concentration in a variety of samples.

Principle

Glutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.

Pictograms

CorrosionHealth hazard
GHS05,GHS08

Signal Word

Danger

Hazard Statements

H314 - H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Developing medications targeting glutamatergic dysfunction in autism: progress to date.
Fung L K and Antonio Y H
CNS Drugs, 29(6), 453-463 (2015)
Glutamate as a neurotransmitter in the healthy brain.
Zhou Y and Danbolt N C
Journal of Neural Transmission. General Section, 121(8), 799-817 (2014)
Leena S Knight et al.
Neurochemical research, 42(12), 3490-3503 (2017-09-18)
Betaine (N-trimethylglycine), a common osmolyte, has received attention because of the number of clinical reports associating betaine supplementation with improved cognition, neuroprotection and exercise physiology. However, tissue analyses report little accumulation of betaine in brain tissue despite the presence of
Glutamate, at the interface between amino acid and carbohydrate metabolism.
Brosnan J T.
The Journal of Nutrition, 130(4), 988S-990S (2000)
The GluN1/GluN2B NMDA receptor and metabotropic glutamate receptor 1 negative allosteric modulator has enhanced neuroprotection in a rat subarachnoid hemorrhage model.
Zhang Z, et al.
Experimental Neurology, 301, 13-25 (2018)
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Articles

Glutamine Metabolism is Dysregulated in Many Cancer Cells

Sigma-Aldrich presents an article about how proliferatively active cells require both a source of carbon and of nitrogen for the synthesis of macromolecules. Although a large proportion of tumor cells utilize aerobic glycolysis and shunt metabolites away from mitochondrial oxidative phosphorylation, many tumor cells exhibit increased mitochondrial activity.

Protocols

Enzymatic Glutamate Assay

Assay protocol for the colorimetric detection of Glutamate in biological samples using the Glutamate assay kit.

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