I2411

Isocitrate Dehydrogenase 1 (NADP+) human

recombinant, expressed in Sf9 cells, ≥90% (SDS-PAGE)

• Synonym(s): IDH1, Isocitrate Dehydrogenase Cytoplasmic
• CAS Number: 9028-48-2
• Enzyme Commission number: 1.1.1.42 (BRENDA, IUBMB)
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in Sf9 cells
• Assay: ≥90% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: 47.6 kDa
• concentration: ≥0.30 mg/mL protein (Bradford)
• NCBI accession no.: NM_005896
• relevant disease(s): cancer (lung)
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, full length [amino acids 1-414 (end)] with C-terminal FLAG-tag, expressed in Sf9 cells via a baculovirus expression system.

Application

Human Isocitrate Dehydrogenase (IDH1) is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. It may be useful as a biomarker for diagnosis and prognosis prediction of non-small cell lung cancer .

Biochem/physiol Actions

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, is a full length amino acid with a C-terminal FLAG-tag and is expressed in Sf9 cells via a baculovirus expression system. IDH1 expression has been correlated with poor survival of non-small cell lung cancer. IDH1 promotes tumor growth .

Physical form

Solution in 45 mM Tris-HCl, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml FLAG peptide, 3 mM DTT, and 10% glycerol at pH 8.0.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable