H1387

Hanks′ Balanced Salts

Modified, without phenol red and sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): HBSS
• MDL number: MFCD00217418
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 500
• form: powder
• technique(s): cell culture | mammalian: suitable
• storage temp.: 2-8°C

Description

General description

Balanced salt solution (BSS) serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It also provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. BSS when combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism. In addition, it also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salts has been used:

• for diluting digested tissues during isolation of pancreatic blood vessels
• for washing mouse intestinal mucosal cells before lysis and sonication
• as an immersion solution to test the in vitro bioactivity of poly(ε-caprolactone)-coated hydroxyapatite scaffold derived from cuttlefish bone

Quantity

Formulated to contain 9.7 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate. Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves