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GE29-0181-82

HiPrep™ Q High Performance 16/10

Cytiva 29-0181-82, pack of 1 column

Synonym(s):

HiPrep Q Column, High Performance Q Column

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

quaternary amine

description

Ion Exchanger Type (value)

packaging

pack of 1 column

manufacturer/tradename

Cytiva 29-0181-82

parameter

1-5 ml/min flow rate
44 psi

bed size

16 mm × 100 mm

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

24-44 μm

avg. part. size

34 μm

cleaning

1-14

working range

2-12

suitability

suitable for bioprocess medium

General description

HiPrep Q HP is a 20 mL strong anion exchange chromatography column for high resolution small-scale protein purification. The Q functional group is a quaternary amino group coupled to the matrix via chemically stable ether linkages. Q Sepharose High Performance is a strong anion exchanger charged with high loading capacity over broad pH ranges.

Features and Benefits

  • Packed with Q Sepharose High Performance strong quarternary ammonium anion exchange resin.
  • Small (34 µm) bead size delivers high-performance, high-resolution purifications.
  • 20 mL HiPrep column format compatible with single-pump configurations and chromatography systems such as ÄKTA systems.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

Protocols

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service