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GE17-1180-01

Resource™ S

Cytiva 17-1180-01, pack of 6 mL

Synonym(s):

Resource S Column

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About This Item

UNSPSC Code:
47101511
NACRES:
NA.56

ligand

sulfonate group

description

Ion Exchanger Type (value)

packaging

pack of 6 mL

manufacturer/tradename

Cytiva 17-1180-01

parameter

87 psi

bed size

16 mm × 30 mm

bed volume

6 mL

matrix

polystyrene/divinylbenzene

avg. part. size

15 μm

particle size

15-15 μm

cleaning

1-14

working range

2-13

suitability

suitable for bioprocess medium (BioProcess medium. Columns not suitable for use with ÄKTAprime plus system.)
suitable for bioprocess medium

General description

RESOURCE™ S columns are prepacked with SOURCE™ 15S, a strong cation exchanger for intermediate lab-scale purification and large-scale polishing of biomolecules with ion exchange chromatography.

The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.

Features and Benefits

  • SOURCE™ 15Q and 15S media are well suited for fast, high-resolution purifications and easy scale-up.
  • Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
  • RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
  • Improved capacity compared to MonoBeads but with a slightly lower resolution.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Practical Considerations for IEX Separation

This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.

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Protocols

SOURCE™: Purification at High Throughput with High Resolution and Easy Scale-Up

This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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