Recommended Products
product name
A373-C6, 97011321
biological source
human skin
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
IL-1 receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human malignant melanoma IL-1 sensitive
Cell Line Description
The cell line A375-C6 has been shown to be sensitive to the IL-1 mediated anti-proliferative effect. It has been derived from the parental line A375 (Sigma Catalogue number. 88113005) by limiting dilution together with its twin clone A375-C5 (Sigma Catalogue number. 97011320) which is IL-1 unresponsive. A375-C6 could be useful for a growth inhibition assay for IL-1. The cells have been eradicated from mycoplasma at ECACC.
Application
IL-1 growth inhibition assay, IL-1 receptor analysis
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 1-3 x 10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service