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Key Documents

ATC-10 (Aedes aegypti)

87091801

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About This Item

UNSPSC Code:
41106514

biological source

mosquito larvae

growth mode

Adherent aggregates

karyotype

2n = 6

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mosquito larvae

Cell Line Description

Derived from pooled newly hatched Aedes aegypti larvae. The cells are susceptible to a range of arboviruses.

Application

Virus studies: Arboviruses

Culture Medium

Mitsuhashi and Maramorosch Insect Medium (pH 7.0) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5 x10,000 cells/cm2 using 0.25% trypsin for 5 mins only 28°C. After quenching the trypsin with fresh mediumdislodge the cells by gentle scraping.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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