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biological source
mouse adipose tissue
growth mode
Adherent
karyotype
Normal diploid
morphology
Fibroblast-like
receptors
Sca-1, c-kit/CD117, nestin, nucleostemin, CD44 and CD106
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Cell Line Origin
Adipose cell line, mouse subcutaneous, mesenchymal, multipotent
Cell Line Description
m17.ASC is a spontaneously immortalized multipotent mesenchymal cell line capable of acquiring adipocyte, chondrocyte, osteocyte and cardiomyocyte phenotypes when cultured with appropriate differentiation factors. m17.ASC provides a valuable tool for in vitro biomimetic studies; assessing growth technologies such as 3-D structures, biochemical cues (selected growth factors and hormones) and physical forces (electrical, mechanical and topographical). It can be used to screen compounds that induce or inhibit proliferation and/or differentiation in tissue specific phenotypes.
DNA Profile
Not specified
Culture Medium
Subculture Routine
Split sub-confluent cultures (90%) approximately 1:6 with media changes or subculture every 2-3 days seeding at 0.5-4 x 104 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37 °C.
Other Notes
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Certificates of Analysis (COA)
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