C3667
Chondroitinase ABC from Proteus vulgaris
BSA free, lyophilized powder, specific activity 50-250 units/mg protein
Synonym(s):
4.2.2.20, 4.2.2.21, Chondroitin ABC Lyase
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About This Item
biological source
bacterial (Proteus vulgaris)
Quality Level
conjugate
(Glucosaminoglycan)
form
lyophilized powder
specific activity
50-250 units/mg protein
mol wt
120 kDa by gel filtration
composition
Protein, ~10% Lowry
solubility
0.01% bovine serum albumin aqueous (BSA) solution: soluble
application(s)
diagnostic assay manufacturing
foreign activity
protease, essentially free
storage temp.
−20°C
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Application
The enzyme has been used to study the consequences of inducing acute, long-lasting changes in chondroitin sulfate proteoglycans using adult rat brain cells. Glycosaminoglycans have been digested with chondroitinase ABC for chondroitin/dermatan sulfate quantitation in keratocyte cell cultures obtained from fresh bovine corneal stromae.
Biochem/physiol Actions
Chondroitinase ABC catalyzes the eliminative degradation of polysaccharides containing (1-4)-β-D-hexosaminyl and (1-3)-β-D-glucuronosyl or (1-3)-α-L-iduronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups. It acts on chondroitin 4-sulfate, chondroitin 6-sulfate, and dermatan sulfate, and acts slowly on hyaluronate. The molecular weight is found to be approximately 120 kDa with 2 non-identical subunits of molecular masses 86 kDa and 32 kDa. The pH optimum is found to be 8.0 with chondroitin sulfate and 6.8 with hyaluronic acid and temperature optimum is 37 °C. 1 mM Zn2+ acts as an inhibitor, while 0.05 M acetate is an activator of the enzyme.
Packaging
Packages based on chondroitinase C activity.
Other Notes
Contains potassium phosphate buffer salts and stabilizer
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Unit Definition
One unit will liberate 1.0 μmole of a mixture of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose and 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate from shark cartilage, per min at pH 8.0 at 37 °C.
Preparation Note
Affinity purified
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Articles
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
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