A8580

Anti-Glutathione-S-Transferase (GST)–Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

• Synonym(s): Anti-Glutathione-S-Transferase, Anti-GST
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: agarose conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: PBS suspension
• mol wt: antigen 27.5 kDa
• should not react with: porcine, bovine, human, rabbit, rat
• technique(s): ELISA: suitable; immunoprecipitation (IP): suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... GSTP1(2950)

Description

General description

Glutathione-S-transferases (GSTs) are dimeric proteins, which belongs to phase II detoxification enzymes family. The highly polymorphic human cytosolic GSTs are divided into six classes, such as, α, μ, ω, π, θ, and ζ.

The antibody is specific for native as well as denatured-reduced forms of glutathione-S-transferase from Schistosoma japonicum. Anti-GST may be used in various immunoassays to identify the expression of GST fusion proteins.

Immunogen

recombinant GST from Schistosoma japonicum expressed in E. coli.

Application

Immunoprecipitation was performed on NIH 3T3 cells transiently transfected with GST fusion proteins. IP was performed using agarose-linked rabbit anti-GST IgG with mixing for 2 hours at 4 degrees.

Rabbit Anti-Glutathione-S-Transferase (GST)-Agarose antibody can be used for ELISA, immunoaffinity purification and immunoprecipitation assays.

Biochem/physiol Actions

Glutathione-S-transferases (GSTs) can catalyse the conjugation of glutathione (GSH) to various endogenous and exogenous electrophilic compounds. The π and μ classes of GSTs controls the mitogen-activated protein (MAP) kinase pathway. It can also serve as transport proteins.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Prepared by coupling cyanogen bromide-activated agarose at 7-8 mg antibody per mL resin volume.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable