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A3678

Sigma-Aldrich

Ammonium persulfate

for molecular biology, suitable for electrophoresis, ≥98%

Synonym(s):

AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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About This Item

Linear Formula:
(NH4)2S2O8
CAS Number:
7727-54-0
Molecular Weight:
228.20
EC Number:
231-786-5
MDL number:
MFCD00003390
UNSPSC Code:
41105319
eCl@ss:
38030421
PubChem Substance ID:
24890800
NACRES:
NB.22

grade

for molecular biology

Quality Level

200

vapor density

7.9 (vs air)

Assay

≥98%

form

powder

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl-): <10 ppm

cation traces

Fe: <10 ppm
heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected
Protease, none detected
RNAse, none detected

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

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General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.
Catalyst for acrylamide gel polymerization.

Analysis Note

Tested for use in acrylamide polymerization.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Maurer HR
Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis (1978)
Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions.
Chiang MY et al.
Biomaterials, 34, 9754-9754 (2013)
Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light.
Fancy DA and Kodadek T
Proceedings of the National Academy of Sciences of the USA, 96, 6020-6020 (1999)
Composite three-dimensional woven scaffolds with interpenetrating network hydrogels to create functional synthetic articular cartilage.
Liao IC et al.
Advances in Functional Materials, 23, 5833-5833 (2013)
Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis.
Alvarez-Gonzalez CA, et al.
Fish Physiology and Biochemistry, 36, 29-29 (2010)
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