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A2350

Sigma-Aldrich

AlumaSeal® II film

non-sterile

Synonym(s):

Sealing film

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10 MG
CZK 8,580.00
25 MG
CZK 23,100.00

CZK 8,580.00


Please contact Customer Service for Availability


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10 MG
CZK 8,580.00
25 MG
CZK 23,100.00

About This Item

UNSPSC Code:
41122410
NACRES:
NB.15

CZK 8,580.00


Please contact Customer Service for Availability

material

aluminum film

sterility

non-sterile

feature

piercable

manufacturer/tradename

Excel Scientific AF-100

packaging

pkg of one zippered bag of 100

parameter

-80 to +120 °C temp. range

technique(s)

PCR: suitable

L

146.1 mm

W × L

8.26 cm × 14.61 cm

W

82.6 mm

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This Item
I4375T9375A5763
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

powder

form

powder

form

powder

assay

≥92.5%

assay

≥96%

assay

≥96% (HPLC)

assay

≥96%

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless to very faintly yellow

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless

solubility

water: 50 mg/mL, clear to very slightly hazy, colorless

solubility

water: 25 mg/mL, clear, colorless to very faintly yellow

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

A soft aluminum foil film designed for the sealing of PCR plates minimizing evaporation and vapor loss during thermal cycling. The film pierces very easily for direct sample recovery with a multichannel pipettor or robotic metal probes without risk of aerosols, cross-contamination or significant gumming of adhesive. An extremely strong, medical-grade adhesive provides a uniform, complete seal which can withstand PCR thermal cycling. Recommended for temperatures from -80 °C to 120 °C, measures 82.6 mm x 146.1 mm and offers sufficient sealing area for all PCR plates. A 36 μm non-permeable soft aluminum foil film eliminates the need for heat-sealing devices or mats during thermal cycling. The proprietary adhesive can withstand harsh chemicals, solvents and reagents. Two end tabs for easy and accurate positioning and labeling.

Legal Information

AlumaSeal is a registered trademark of Excel Scientific, Inc.

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    Protocols

    Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

    Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

    Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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