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54287

Sigma-Aldrich

Anti-Rabbit IgG−Abberior® CAGE 590 antibody produced in goat

for single-molecule switching microscopy (e.g. PALM, STORM, GSDIM)

Synonym(s):

Abberior® CAGE 590-Anti-Rabbit IgG antibody produced in goat

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About This Item

UNSPSC Code:
12352200

biological source

goat

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rabbit

concentration

~1 mg/mL

fluorescence

λex 595 nm; λem 620 nm in PBS, pH 7.4 (photoactivated)

storage temp.

−20°C

General description

Abberior CAGE 590 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent orange-red dye.
It can most effectively be excited from 560 to 600 nm. Abberior CAGE 590 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. Further, after photoactivation, Abberior CAGE 590 can be tracked in confocal and epifluorescence microscopy to analyses molecular dynamics such as diffusion, flow directions and velocities, e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 595 (photoactivated, PBS pH 7.4), 351 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 75 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 615 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, t [ns]: 4,0 (photoactivated, PBS pH 7.4)

Suitability

Designed and tested for fluorescent super-resolution microscopy

Analysis Note

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Other Notes

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Legal Information

abberior is a registered trademark of Abberior GmbH

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related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCBR5328V
BCBQ6710V

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Rhodamines NN: a novel class of caged fluorescent dyes.
Vladimir N Belov et al.
Angewandte Chemie (International ed. in English), 49(20), 3520-3523 (2010-04-15)

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