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Atto 633 Protein Labeling Kit

Name: Atto 633 Protein Labeling Kit
Brand: SIGMA

BioReagent, suitable for fluorescence

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About This Item

EC Number:

200-664-3

UNSPSC Code:

12352200

NACRES:

NA.32

product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λ_ex 633 nm; λ_em 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

suitability

suitable for fluorescence

storage temp.

2-8°C

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General description

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Application

Atto 633 is a red-emitting fluorescence label with strong absorption, high quantum yield (64%), high photostability, good water solubility, and very little triplet formation. This label is optimized for use with diode laser excitation at 633 nm and characterized by high photostability.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

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satFRET: estimation of Förster resonance energy transfer by acceptor saturation.

Martin Beutler et al.

European biophysics journal : EBJ, 38(1), 69-82 (2008-09-05)

We demonstrate theoretically and experimentally the quantification of Förster resonance energy transfer (FRET) by direct and systematic saturation of the excited state of acceptor molecules. This version of acceptor depletion methods for FRET estimation, denoted as "satFRET" is reversible and

Home-built integrated microarray system (IMAS). A three-laser confocal fluorescence scanner coupled with a microarray printer.

Sotirios S Tragoulias et al.

Analytical and bioanalytical chemistry, 390(6), 1563-1573 (2008-01-30)

Microarray technology covers the urgent need to exploit the accumulated genetic information from large-scale sequencing projects and facilitate investigations on a genome-wide scale. Although most applications focus on DNA microarrays, the technology has expanded to microarrays of proteins, peptides, carbohydrates

Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates.

Judith E Berlier et al.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 51(12), 1699-1712 (2003-11-19)

Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were

Articles

Atto Dyes and Tracy Dyes for Fluorescent Protein Labeling

Sigma® offers protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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