137265

HEPES sodium salt

EMPROVE^® EVOLVE

• Synonym(s): HEPES, Sodium Salt, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS Number: 75277-39-3
• Molecular Weight: 260.29
• MDL number: MFCD00036463
• UNSPSC Code: 12161700

Properties

• biological source: synthetic
• Quality Level: 400
• product line: EMPROVE^® EVOLVE
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: Endotoxin, microbial, and trace metals; tested
• pH: 6.8-8.2
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• pKa: 7.5
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• storage temp.: 2-30°C
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

Application

HEPES Sodium is a biological buffer often referred to as a “Good′s” buffer. HEPES Sodium, used in conjunction with HEPES, is the most commonly used biological buffer formulation since the pKa of the buffer is 7.31 which corresponds to the physiological pH of 7.36. HEPES Sodium is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES Sodium is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagent formulations.

Legal Information

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable