67515

STEC Millichrome^™ plus Supplement

suitable for microbiology

• Synonym(s): Shiga-Toxin producing E. coli (STEC) Chromogenic supplement
• UNSPSC Code: 41171614
• NACRES: NA.74

Properties

• sterility: aseptically filled
• Quality Level: 200
• form: dry powder
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbiological culture: suitable
• solubility: sterile water: 1 (vial/10ml)
• application(s): food and beverages; microbiology
• shipped in: ambient
• storage temp.: 2-8°C
• suitability: selective for bacteria: Escherichia coli (Shiga Toxin-STEC

Description

General description

STEC Millichrome^™ plus Supplement is a selective media supplement for the detection of E. coli (STEC). The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of E. coli (STEC).Millichrome^™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 5 vials, each vial sufficient for 1 liter of final media. One vial of the supplement should be rehydrated with 10 mL of sterile water and then added to 1 liter of melted STEC Millichrome^™ plus Agar base cooled at 45-50 °C.

Application

STEC Millichrome^™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation and presumptive identification of E. coli (STEC) from food, animal feed, and environmental samples.

Packaging

5 vials (1 vial is sufficient for 1 L of final media)

Preparation Note

• Aseptically rehydrate 1 vial with 10 mL of sterile water.
• Swirl well until complete dissolution.
• Add 1 vial rehydrated solution to 1L STEC Millichrome^® plus Agar base cooled at 45-50 °C.
• Swirl gently to homogenize.

Analysis Note

The Shiga toxins E. coli is detected by a characteristic mauve color after only 24 h at an optimal growth temperature of 37°C, while most other Enterobacteriaceae form Colorless, blue, or inhibited after incubation at the same temperature

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3