11721933001
Roche
DNA Molecular Weight Marker XIV (100 bp ladder)
solution, pkg of 50 μg (in 200 μl)
Synonym(s):
DNA marker
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About This Item
UNSPSC Code:
41105335
form
solution
Quality Level
packaging
pkg of 50 μg (in 200 μl)
manufacturer/tradename
Roche
concentration
250 μg/mL
color
colorless
solubility
water: miscible
storage temp.
−20°C
General description
Fragment mixture prepared by cleavage of a specially constructed plasmid with restriction endonucleases.
Size Range: 100 to 1500 bp and an additional band of 2642 bp
Size Range: 100 to 1500 bp and an additional band of 2642 bp
Application
DNA Molecular Weight Marker XIV (100 bp ladder) allows accurate sizing of DNA fragments generated by PCR or restriction digest or RT-PCR, following separation on agarose gels. The DNA molecular weight marker can be used in conjunction with DNA Molecular Weight Marker XIII (50 bp ladder) for precise size determination.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
Electrophoretic separation of this molecular weight marker results in a regular pattern. The fragments have 5′-protruding ends and can be labeled with radioactive nucleotides (e.g.,32P-dTTP or 32P-dGTP) by standard filling-in reactions. End-labeling reactions can be performed with a radioactive or nonradioactive dideoxynucleotide (e.g., DIG-11-ddUTP) and Terminal Transferase.
DNA Molecular Weight Marker XIV has been used in gel electrophoresis.
Sequence
The mixture contains 15 double-stranded DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1100, 1200, 1300, 1400, 1500, and an additional band of 2642 bp. The 500-bp and 1000-bp banding pattern is two to three times brighter.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.To make them easy to identify, the 500 and 1,000 bp bands are two to three times as bright as the other bands in the ladder.
Unit Definition
50 μg = 1A260 unit
Physical form
Ready-to-use solution, 250 μg/ml, in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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