MABT36

Anti-COMP (Thrombospondin-5) Antibody, clone 484D1

clone 484D1, from rat

• Synonym(s): Cartilage oligomeric matrix protein, Thrombospondin-5
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 484D1, monoclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000086
• UniProt accession no.: P49747
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... COMP(1311)

Description

General description

Cartilage oligomeric matrix protein (COMP, Thrombospondin-5, TSP5) is a member of the thrombospondin gene family. COMP is usually found in the extracellular matrix and is regularly linked to cartilage tissue. COMP seems to protect chondrocytes against cell death, whether tumor necrosis factor alpha is present or not. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 and pseudoachondroplasia.

Immunogen

Human cartilage oligometric matrix recombinant protein.

Application

Immunohistochemistry (Frozen Tissue) Analysis: A previous lot of this antibody was shown to detect COMP in osteoblasts lining the bone spicules of adult human subchondral bone section (Di Cesare, P.E., et al. (2000). Journal of Orthopaedic Research. 18:713.720).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-COMP (Thrombospondin-5) Antibody, clone 484D1 is validated for use in WB, IH for the detection of COMP (Thrombospondin-5).

Quality

Evaluated by Western Blot in human chrondrocytes cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected COMP in 10 µg of human chrondrocyte cell lysate.

Target description

~83 kDa Observed

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human chrondrocyte cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable