MABS21

Anti-PORCN Antibody, clone 15G12.1

clone 15G12.1, from mouse

• Synonym(s): Probable protein-cysteine N-palmitoyltransferase porcupine, Protein MG61
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 15G12.1, monoclonal
• species reactivity: rat, mouse, human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_073736
• UniProt accession no.: Q9H237
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Porcn(53627)

Description

General description

PORCN is a multi-pass endoplasmic reticulum protein involved in the processing of wingless and other Wnt proteins that facilitate their secretion from Wnt-producing cells. Studies have shown mutations in the genes that encode for PORCN cause a pleiotropic X-linked dominant disorder, focal dermal hypolasia (FDH, also known as Goltz syndrome). Skeletal abnormalities such as syndactyly, ectordactyly, and brachydactyly, and some cases of osteopathia striata have also been attibuted to PORCN abnormalities.

Immunogen

Linear peptide corresponding to human PORCN.

Application

Anti-PORCN Antibody, clone 15G12.1 is an antibody against PORCN for use in WB & IC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PORCN in HeLa and A431 cells.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected PORCN in 10 µg of mouse brain tissue lysate.

Target description

~62 kDa observed.
Calculated molecular weight of 52 kDa The actual MW should be observed at ~45-65 kDa Helical membrane protein migration on SDS-PAGE may not exactly correlate with the calculated molecular weights (PNAS, 2009, 106:1760-1765).

Physical form

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable