MABS194
Anti-c-K-Ras Antibody, clone 234-4.2
clone 234-4.2, from mouse
Synonym(s):
GTPase Kras, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
234-4.2, monoclonal
species reactivity
human
species reactivity (predicted by homology)
rat (immunogen homology)
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... KRAS(3845)
General description
GTPase KRas (c-k-ras; K-ras) is a membrane-bound G protein belonging to the family of Ras GTPases. They are activated by the binding of GTP in response to signals from various receptors including the epidermal growth factor receptor (EGFR). Ras GTPases are regulated by guanine nucleotide exchange factors (GEF) and GTPase activating proteins (GAP) and lie upstream of the MAP kinase pathway which controls a range of cellular processes including proliferation and differentiation. Previous studies have suggested that KRas plays an important role in embryonic development, and it may also contribute to the pathogenesis of colorectal cancer via an EGFR-mediated pathway.
Immunogen
Recombinant protein corresponding to rat c-K-Ras.
Application
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-c-K-Ras Antibody, clone 234-4.2 is validated for use in Western Blotting, IHC for the detection of c-K-Ras.
Western Blot Analysis: A representative lot detected c-K-Ras in A431 cell lysate.
Western Blot Analysis: A representative lot from an independent laboratory detected c-K-Ras in primary human
lymphoid leukemia cell lysates, and in normal and HA-dynamin K44A mutant HeLa cell lysates (Omerovic, J., et al. (2008). Oncogene. 27(19):2754-2762.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected c-K-Ras in non-small cell lung cancer cells (Volm, M., et al. (2002). Clin Cancer Res. 8(6):1843-1848.).
Western Blot Analysis: A representative lot from an independent laboratory detected c-K-Ras in primary human
lymphoid leukemia cell lysates, and in normal and HA-dynamin K44A mutant HeLa cell lysates (Omerovic, J., et al. (2008). Oncogene. 27(19):2754-2762.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected c-K-Ras in non-small cell lung cancer cells (Volm, M., et al. (2002). Clin Cancer Res. 8(6):1843-1848.).
Quality
Evaluated by Western Blot in SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected c-K-Ras in 10 µg of SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected c-K-Ras in 10 µg of SW-480 cell lysate.
Target description
~22 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
SW-480 cell lysate
SW-480 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ras isoform abundance and signalling in human cancer cell lines.
Omerovic, J, et al.
Oncogene, 27, 2754-2762 (2008)
Elaine Lai-Han Leung et al.
International journal of cancer, 145(5), 1334-1345 (2019-02-21)
Oncogenic KRAS is considered a promising target for anti-cancer therapy. However, direct pharmacological strategies targeting KRAS-driven cancers remained unavailable. The prenyl-binding protein PDEδ, a transporter of KRAS, has been identified as a potential target for pharmacological inhibitor by selectively binding
Expression profile of genes in non-small cell lung carcinomas from long-term surviving patients.
Volm, Manfred, et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 8, 1843-1848 (2002)
Enkhtsetseg Munkhbaatar et al.
Nature communications, 11(1), 4527-4527 (2020-09-12)
Evasion of programmed cell death represents a critical form of oncogene addiction in cancer cells. Understanding the molecular mechanisms underpinning cancer cell survival despite the oncogenic stress could provide a molecular basis for potential therapeutic interventions. Here we explore the
Almas Yaqoob et al.
PloS one, 15(5), e0231948-e0231948 (2020-05-06)
In our search for bioactive mushrooms native to British Columbia, we determined that the ethanol extracts from fruiting bodies of the terrestrial polypore Albatrellus flettii had potent anti-cell viability activity. Using bioassay-guided fractionation, mass spectrometry and nuclear magnetic resonance, we
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