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MABN92-AF647

Sigma-Aldrich

Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor™ 647 Conjugate

clone 20A12.1, from mouse, ALEXA FLUOR™ 647

Synonym(s):

Allograft inflammatory factor 1, AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.45

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 647

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

20A12.1, monoclonal

species reactivity

mouse, human, rat

technique(s)

immunofluorescence: suitable

isotype

IgG1κ

NCBI accession no.

NP_001614

UniProt accession no.

P55008

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... AIF1(199)

General description

Allograft inflammatory factor 1 (UniProt P55008; also known as AIF-1, Daintain, Interferon gamma responsive transcript, Ionized calcium-binding adapter molecule 1, Protein G1) is encoded by the AIF1 (also known as G1, IBA1) gene (Gene ID 199) in human. Iba1/AIF1 is a 17 kDa interferon-gamma- (IFN-gamma-) inducible, Ca(2+)-binding EF-hand protein. Iba1/AIF1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and macrophage activation/function. It is also shown to be expressed in microglial cells in autominnume disesase models such as experimental autoimmune encephalomyelitis, neuritis and uveitis.

Application

Anti-Iba1/AIF1 Antibody, clone 20A12.1, Alexa Fluor™ 647 Conjugate is an antibody against AIF1 for use in Immunofluorescence.
The unconjugated antibody (Cat. No. MABN92) is shown to be suitable also for Western blotting and immunohistochemistry applications.

Quality

Evaluated by Immunofluorescence in human spleen tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected Iba1/AIF1 in human spleen tissue.

Target description

~17 kDa observed.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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