MABN893
Anti-ABCA1 Antibody, clone MABI98-7
clone MAB198-7, from rat
Synonym(s):
ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1, ATP-binding cassette 1, ABCA 1, ABCA-1
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MAB198-7, monoclonal
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Abca1(535379)
Related Categories
General description
ATP-binding cassette sub-family A member 1, also known as ATP-binding cassette transporter 1 (ABC-1), or ATP-binding cassette 1, and encoded by the gene name Abca1 or Abc1, is a cAMP-dependent and sulfonylurea-sensitive anion transporter. ABCA1 is a member of the ABC transporter superfamily and the ABCA family. ABCA1 is a key gatekeeper influencing intracellular cholesterol transport. ABCA1 has two homologous halves, each containing a hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain, and may act as a multifunctional polypeptide. ABCA1 has been shown to be down-regulated by endotoxins (LPS) or cytokines (TNF and IL-1) in J774 macrophages. The down-regulation by endotoxin in macrophages is not likely to be mediated by the liver X receptor/retinoic X receptor (LXR/RXR). ABCA1 interacts with MEGF10 and is widely expressed in adult tissues, with the highest levels found in uterus and pregnant uterus. Mutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking.
Immunogen
Epitope: Immunogen is near C-terminal.
Linear peptide corresponding to the C-terminus of Mouse ABCA1.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
This Anti-ABCA1 antibody is validated for use in WB, IH for the detection of ABCA1.
Western Blotting Analysis: A representative lot of MABN893 deteted ABCA1 in lysate of WT mouse brain and demonstrated a loss of signal in lysates of ABCA1 knock out adult mouse brain (Hu et al., 2008. J Lipid Res. 2008 Feb;49(2):386-93.)
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ABCA1 in human cerebral cortex, thalamus, and kidney tissues.
Produced in collaboration with MAB Institute, Inc.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ABCA1 in human cerebral cortex, thalamus, and kidney tissues.
Produced in collaboration with MAB Institute, Inc.
Quality
Evaluated by Western Blotting in Calyculin and Okadaic Acid treated NIH-3T3 cell lysate.
Western Blotting Analysis: A 1 µg/mL diultion of this antibody detected ABCA1 in 10 µg of Calyculin and Okadaic Acid treated NIH-3T3 cell lysate.
Western Blotting Analysis: A 1 µg/mL diultion of this antibody detected ABCA1 in 10 µg of Calyculin and Okadaic Acid treated NIH-3T3 cell lysate.
Target description
~254 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing PBS with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Noriyuki Iwamoto et al.
Arteriosclerosis, thrombosis, and vascular biology, 30(7), 1446-1452 (2010-04-17)
To investigate the interaction of ATP-binding cassette transporter A1 (ABCA1) with calmodulin in relation to its calpain-mediated degradation because many calpain substrates bind calmodulin to regulate cellular functions. The activity of ABCA1 is regulated through proteolysis by calpain. An immunoprecipitation
Wei Hu et al.
Journal of lipid research, 49(2), 386-393 (2007-11-24)
Serum amyloid A (SAA) was markedly increased in the plasma and in the liver upon acute inflammation induced by intraperitoneal injection of lipopolysaccharide (LPS) in mice, and SAA in the plasma was exclusively associated with HDL. In contrast, no HDL
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service