MABE1123
Anti-XPB Antibody, clone 15TF2-1B3
ascites fluid, clone 15TF2-1B3, from mouse
Synonym(s):
TFIIH basal transcription factor complex helicase XPB subunit, Basic transcription factor 2 89 kDa subunit, BTF2 p89, DNA excision repair protein ERCC-3, DNA repair protein complementing XP-B cells, TFIIH basal transcription factor complex 89 kDa subunit
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
15TF2-1B3, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ERCC3(2071)
General description
Transcription factor II human (TFIIH) basal transcription factor complex helicase XPB subunit (EC 3.6.4.12; UniProt P19447; also known as BTF2 p89, DNA excision repair protein ERCC-3, DNA repair helicase, DNA repair protein complementing XP-B cells, TFIIH 89 kDa subunit, TFIIH basal transcription factor complex 89 kDa subunit, TFIIH p89, Basic transcription factor 2 89 kDa subunit, Xeroderma pigmentosum group B-complementing protein) is encoded by the ERCC3 (also known as BTF2, GTF2H, RAD25, TFIIH, XPB) gene (Gene ID 2071) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by TFIIH. The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.
Immunogen
Epitope: N-terminus
Recombinant protein corresponding to human XPB.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-XPB Antibody, clone 15TF2-1B3 is validated for use in Western Blotting, Immunocytochemistry for the detection of XPB.
Western Blotting Analysis: A representative lot detected Xpb in murine embryonic fibroblasts (MEFs) and HeLa cells, as well as in transgenic animal-derived MEFs expressing Xpb lacking last 43 C-terminal amino acids (Andressoo, J.O., et al. (2009). Mol Cell Biol.29(5):1276-290).
Western Blotting Analysis: A representative lot detected endougenous as well as exogenously expressed Xpb in both U2OS17 whole cell lysate and in THIIF p62 subunit immunoprecipitate (Ziani, S., et al. (2014). J Cell Biol.;206(5):589-598).
Western Blotting Analysis: A representative lot detected Xpb in THIIF TTDA subunit immunoprecipitate (Giglia-Mari,G., et al. (2006). PLoS Biol. 4(6): e156).
Immunocytochemistry Analysis: A representative lot detected XPB recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).
Western Blotting Analysis: A representative lot detected endougenous as well as exogenously expressed Xpb in both U2OS17 whole cell lysate and in THIIF p62 subunit immunoprecipitate (Ziani, S., et al. (2014). J Cell Biol.;206(5):589-598).
Western Blotting Analysis: A representative lot detected Xpb in THIIF TTDA subunit immunoprecipitate (Giglia-Mari,G., et al. (2006). PLoS Biol. 4(6): e156).
Immunocytochemistry Analysis: A representative lot detected XPB recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).
Quality
Evaluated by Western Blotting in HeLa nuclear extract.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPB in 10 µg of HeLa nuclear extract.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPB in 10 µg of HeLa nuclear extract.
Target description
~85 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Sequential and ordered assembly of a large DNA repair complex on undamaged chromatin.
Ziani, S; Nagy, Z; Alekseev, S; Soutoglou, E; Egly, JM; Coin, F
The Journal of cell biology null
An Xpb mouse model for combined xeroderma pigmentosum and cockayne syndrome reveals progeroid features upon further attenuation of DNA repair.
Andressoo, JO; Weeda, G; de Wit, J; Mitchell, JR; Beems, RB; van Steeg, H; van der Horst et al.
Molecular and cellular biology null
Sylvain Geny et al.
Methods in molecular biology (Clifton, N.J.), 2247, 39-57 (2020-12-11)
Macromolecular complexes govern the majority of biological processes and are of great biomedical relevance as factors that perturb interaction networks underlie a number of diseases, and inhibition of protein-protein interactions is a common strategy in drug discovery. Genome editing technologies
Giuseppina Giglia-Mari et al.
PLoS biology, 4(6), e156-e156 (2006-05-04)
Transcription/repair factor IIH (TFIIH) is essential for RNA polymerase II transcription and nucleotide excision repair (NER). This multi-subunit complex consists of ten polypeptides, including the recently identified small 8-kDa trichothiodystrophy group A (TTDA)/ hTFB5 protein. Patients belonging to the rare
Sergey Alekseev et al.
Chemistry & biology, 21(3), 398-407 (2014-02-11)
Nucleotide excision repair (NER) removes DNA lesions resulting from exposure to UV irradiation or chemical agents such as platinum-based drugs used as anticancer molecules. Pharmacological inhibition of NER is expected to enhance chemosensitivity but nontoxic NER inhibitors are rare. Using
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