427211

Lactate Dehydrogenase, Porcine Heart

• Synonym(s): Lactate Dehydrogenase, Porcine Heart
• CAS Number: 9001-60-9
• Enzyme Commission number: 1.1.1.27 (BRENDA, IUBMB)
• MDL number: MFCD00131466
• UNSPSC Code: 12352202

Properties

• form: lyophilized
• Quality Level: 100
• specific activity: ≥300 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• impurities: ≤0.01% PK, MK; ≤0.03% GPT, GOT; ≤0.1% MDH
• pI: 4.6
• solubility: aqueous buffer: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Has an optimal pH of 7.0 and a pI of 4.6. Note: 1KU = 1000 units.

Native lactate dehydrogenase from porcine heart. A tetrameric enzyme that catalyzes the reversible conversion of pyruvate to lactate. The overall equilibrium of this reaction favors lactate formation. Note: 1KU = 1000 units.

Note: 1 KU = 1000 units

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cause the oxidation of one micromole of NADH per minute at 25°C, pH 7.4

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.4.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 24 h at 4°C. Do not freeze solutions.

Other Notes

Kornberg, A. 1995. Methods Enzymol.1, 441

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable