1.52022
Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 100 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® HighResolution RP-18 endcapped 100-4.6 HPLC column
About This Item
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product name
Chromolith® HighResolution RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column
material
PEEK column
Quality Level
Agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
endcapped
packaging
1 ea of
extent of labeling
15% Carbon loading
18% Carbon loading
parameter
200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.
technique(s)
HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)
column L × I.D.
10 cm × 4.6 mm
surface area
250 m2/g × 250 m2/g
matrix
highly porous
monolithic silica
monolithic silica
matrix active group
C18 bonding phase, octadecylsilane (ODS)
C18 bonding phase
pore size
1.15 μm macroporosity
1.5 μm macroporosity
15 nm mesoporosity
15 nm mesoporosity
operating pH range
2-7.5
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
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General description
Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for high resolution separations of complex compound mixtures on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns
Application
- A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples.: This study, conducted by Levent et al., introduces a highly sensitive LC-MS/MS method for quantifying ceftiofur in pharmaceutical preparations and milk samples. The method utilizes Chromolith® HighResolution RP-18 endcapped columns, showcasing their application in enhancing the resolution and sensitivity of chromatographic analysis in complex matrices (Levent et al., 2021).
Analysis Note
Pressure: 40 - 70 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 140000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.90 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35
Legal Information
Storage Class Code
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Protocols
Praziquantel Determination in Aquarium Water- Learn how the use of a Chromolith® monolithic silica column allows simplification of sample preparation.
Folic acid or folate is classified as a B vitamin (B9). Folic acid is synthetically produced, and used in fortified foods and supplements. Folate is converted by humans to dihydrofolate (dihydrofolic acid), tetrahydrofolate (tetrahydrofolic acid), and other derivatives, which have various biological activities.
This application illustrate how Rutin can be determined in skin cream samples using a monolithic Chromolith® HighResolution RP-18 endcapped column with UV detection.
Related Content
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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