1.50443
SeQuant™ ZIC®-HILIC 100Å (3.5 µm) HPLC Columns
L × I.D. 250 mm x 2.1 mm HPLC Column, PEEK-lined stainless steel
About This Item
Recommended Products
product name
SeQuant™ ZIC-HILIC™ 3.5µm,100Å 250 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
Agency
suitable for USP L114
product line
SeQuant™
feature
endcapped: no
parameter
550 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
25 cm × 2.1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
100 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
The Pore size of 100 Å makes this column perfectly suitable for the separation of small polar molecules under HILIC conditions.
The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.
Thanks to their favorable retention and robust performance, SeQuant™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant™ ZIC®-HILIC for analysis of melamine and related substances.
SeQuant™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
Analysis Note
Symmetry (Cytosine): 0.95 - 1.40
Legal Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service