1.25195

Cellvento^® 4HEK Powder

Chemically defined, powder, suitable (for HEK293 cell culture)

Properties

• description: shipped with cooling pack
• form: dry powder; powder
• technique(s): cell culture | mammalian: suitable
• suitability: suitable (for HEK293 cell culture)
• storage temp.: 2-8°C

Description

General description

Cellvento^® 4HEK Medium is a chemically defined medium optimized for adeno-associated virus (AAV) production in multiple human embryonic kidney (HEK) 293 lineages. Cellvento^® 4HEK Medium has been streamlined and cell culture media components incorporated to increase media stability and reduce protein oxidation. The formulation is animal component free.

Application

Cellvento^® 4HEK Medium can be used as amplification and production medium for gene therapy applications. This medium enables low doubling time and high viable cell densities with multiple HEK293 cell lines. Cellvento^®4HEK Medium is compatible with commonly used transfection reagents like PEI, supports AAV production and facilitates high titers of full capsids in various HEK293 lineages. This easy-to-use medium can be used for all stages like growth, transfection, and production.

Preparation Note

Dry powder medium must be supplemented with 2.5 g/LSodium Bicarbonate. Once hydrated, medium must be supplemented with 6-10 mML-Glutamine.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319
• Precautionary Statements: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable