1.13724

HPTLC plates, Silica gel 60 RP-18 F₂₅₄s

pkg of 25 plates, plate L × W 10 cm × 10 cm, glass support

• Synonym(s): Reversed Phase High Performance TLC Plates
• UNSPSC Code: 41115711
• NACRES: NB.21

Properties

• material: glass support; silica gel 60 matrix
• Quality Level: 100
• feature: binder Organic Polymer; fluorescent indicator
• packaging: pkg of 25 plates
• technique(s): thin layer chromatography (TLC): suitable
• layer thickness: 200 μm
• plate L × W: 10 cm × 10 cm
• particle size: 5-6 μm
• pore size: 60 Å medium pore diameter
• storage temp.: 2-30°C

Description

General description

25 Glass plates 10 x 10 cm

RP modified plates serve two purposes: act as a pilot method for HPLC and allow to choose various solvent system for special separations. Our RP-modified silica layers are well suited for many separation challenges that unmodified silica cannot overcome. These layers use aqueous solvent systems to separate extremely non-polar substances and analyze particular polar substances that can adapt to ion-pair chromatography. What’s more, RP-modified silica layers are less dependent on atmospheric humidity. Unlike unmodified silica, RP-phases do not exhibit catalytic activity. This makes them the plates of choice for unstable substance that tend to experience oxidative degradation.

Application

Glass HPTLC Silica gel 60 RP-18 F₂₅₄s plates. Silica gel HPTLC plates size 10 x 10 cm, 25 sheets.

Linkage

Replaces: 13724-5; 13724

Analysis Note

Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Cholesterin test
hRf-values
- cholesterin, cholesterin test:
11 - 23
B) Steroid test
- methyltestosterone, steroid test: 20 - 40
- Reichstein S, steroid test: 50 - 70
- hydrocortisone, steroid test: 65 - 85
Typical value determined on an activated plate (120°C, 15 min)
Eluent a) aceton/ water (95/5 v/v)
Eluent b) acetonitrile/ water (70/30 v/v)