1.04008

Formamide

EMPLURA^®

• Synonym(s): Formic acid amide, Methane amide, Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• MDL number: MFCD00007941
• UNSPSC Code: 12352111
• EC Index Number: 200-842-0

Properties

• vapor density: 1.55 (vs air)
• Quality Level: 200
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• product line: EMPLURA^®
• Assay: ≥99% (N)
• form: liquid
• autoignition temp.: 932 °F
• potency: 5325 mg/kg LD₅₀, oral (Rat); 17000 mg/kg LD₅₀, skin (Rabbit)
• expl. lim.: 2.7-19.0 % (v/v)
• technique(s): hybridization: suitable
• impurities: ≤0.3% Water (Karl Fischer)
• refractive index: n20/D 1.447 (lit.)
• pH: 8-10 (20 °C, 200 g/L in H₂O)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• transition temp: flash point 175 °C
• density: 1.134 g/mL at 25 °C (lit.)
• storage temp.: 15-25°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Analysis Note

Assay (N; Kjeldahl): ≥ 99.0 %
Identity (IR): passes test
Density (d 20 °C/ 4 °C): 1.132 - 1.135
Water (according to Karl Fischer): ≤ 0.3 %

Legal Information

EMPLURA is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351 - H360D - H373
• Precautionary Statements: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Target Organs: Blood
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): 305.6 °F
• Flash Point(C): 152 °C