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09-126

Sigma-Aldrich

Anti-Tribbles-1 Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_079471

UniProt accession no.

Q96RU8

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TRIB1(10221)

General description

The Tribbles family of proteins are kinase-like proteins that contain a kinase-like domain, but lack either a catalytic domain or a protein-protein interaction domain (2). They are believed to regulate signal processing for various pathways including MAPK, Akt, and NFkB that are involved in various aspects of development, diabetes, and cancer (1,2,3). Their involvement has been reported to regulate a number of pathways that include mitosis, cell activation, and apoptosis. They are are a family made up of three family members. Tribbles-1 (TRIB1, SKIP1, G-protein-coupled receptor induced protein, and phosphoprotein regulated by mitogenic pathways) is a modulator of MAPK action. It has been shown to directly interact with the upstream kinases MEK1 and MKK4 of Erk and JNK, respectively(1). Tribble-2 (Trib2, TRB2) has been shown to interact with Akt(2). Tribbles-3 (TRIB3, TRB3. NIPK, SKIP3) has been shown to interact with Akt and the transcription factors ATF4 and NFkB p65 (RelA). It is interaction with RelA is thought to be negative regulation. Interestingly, it has been reported to regulate Akt activation via insulin(3).

Specificity

Tribbles-1

Immunogen

KLH-conjugated sythetic linear peptide directed against a region of the N-terminal portion of Tribbles-1.

Application

This Anti-Tribbles-1 Antibody is validated for use in IH, WB for the detection of Tribbles-1.

Quality

routinely evaluated by western blot on lysate from HeLa cells

Target description

41 kDa

Physical form

100 μg in 100μL of affinity purified rabbit serum in storage buffer containing PBS with 0.05% sodium azide.

Analysis Note

Control
HeLa

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hye Youn Sung et al.
Biology, 1(1), 43-57 (2012-01-01)
Development of the atherosclerotic plaque involves a complex interplay between a number of cell types and an extensive inter-cellular communication via cell bound as well as soluble mediators. The family of tribbles proteins has recently been identified as novel controllers
Jessica M Johnston et al.
Science advances, 5(10), eaax9183-eaax9183 (2019-11-07)
Macrophages drive atherosclerotic plaque progression and rupture; hence, attenuating their atherosclerosis-inducing properties holds promise for reducing coronary heart disease (CHD). Recent studies in mouse models have demonstrated that Tribbles 1 (Trib1) regulates macrophage phenotype and shows that Trib1 deficiency increases

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