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Key Documents

05-206

Sigma-Aldrich

Anti-Laminin B2 Antibody, clone A5

clone A5, Upstate®, from rat

Synonym(s):

Anti-LAMS, Anti-NPHS5, Anti-PIERS

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

A5, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG

NCBI accession no.

NM_002292

UniProt accession no.

P55268

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... LAMB2(3913)

Specificity

B2 chain laminin; does not cross-react with other basement membrane components or fibronectin

Immunogen

Murine EHS laminin

Application

Anti-Laminin B2 Antibody, clone A5 is an antibody against Laminin B2 for use in IP, IH.
Not recommended for WB.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Quality

routinely evaluated on unfixed, frozen monkey brain sections

Target description

196 kDa

Physical form

0.1M Tris-glycine, pH 7.0
Format: Purified
Protein G Chromatography

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A V Ljubimov et al.
International journal of cancer, 50(4), 562-566 (1992-02-20)
Double-label immunofluorescence was used to monitor basement-membrane composition and integrity in 22 human colon polyps, 36 adenocarcinomas and 2 metastases. Cryostat sections were stained with polyclonal anti-laminin anti-serum combined with monoclonal antibodies (MAbs) to all major basement-membrane components (laminin, entactin/nidogen
Immunohistochemical distribution of basement membrane proteins in the human inner ear from older subjects.
Akira Ishiyama,Sarah E Mowry,Ivan A Lopez,Gail Ishiyama
Hearing Research null
Benjamin D Cosgrove et al.
Nature medicine, 20(3), 255-264 (2014-02-18)
The elderly often suffer from progressive muscle weakness and regenerative failure. We demonstrate that muscle regeneration is impaired with aging owing in part to a cell-autonomous functional decline in skeletal muscle stem cells (MuSCs). Two-thirds of MuSCs from aged mice
Basement membrane components produced by a mouse ascites teratocarcinoma TB24. Analysis with monoclonal and polyclonal antibodies.
Ljubimov, A V, et al.
Experimental Cell Research, 165, 530-540 (1986)
Andrzej Steplewski et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 34(3), 489-501 (2015-08-05)
Post-traumatic joint contracture is a debilitating consequence of trauma or surgical procedures. It is associated with fibrosis that develops regardless of the nature of initial trauma and results from complex biological processes associated with inflammation and cell activation. These processes
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