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Key Documents

G001835

HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-WAX, 5 cm × 4.6 mm, 5 μm particles (59602-U)
mobile phase: ([A] 20 mM Tris, pH 8.0 with hydrocloric acid; [B] 20 mM Tris, 0.5 M sodium chloride, pH 8.0 with hydrochloric acid)
gradient: 0 to 10% B in 30 min. (linear)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μL

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

Discovery BIO PolyMA-SCX and PolyMA-WAX columns give sharp, efficient peaks for a wide variety of proteins.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
    Superoxide dismutase

standard

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Description
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