ZRB2992

Anti-GPX1 Antibody, clone 1J19 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Cellular glutathione peroxidase, EC:1.11.1.12, EC:1.11.1.9, Phospholipid-hydroperoxide glutathione peroxidase GPX1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1J19, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 22 kDa; observed mol wt ~22 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey, canine, porcine, feline
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_000572.2
• UniProt accession no.: P07203
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... GPX1(2876)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1J19 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects GPX1. It targets an epitope within 15 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human GPX1.

Application

Quality Control Testing

Evaluated by Western Blotting in PC-3 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GPX1 in PC-3 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000-10,000 dilution from a representative lot detected GPX1 in HepG2, THP-1, human liver, and human kidney lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GPX1 in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GPX1 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Glutathione peroxidase 1 (UniProt: P07203; EC:1.11.1.9; EC:1.11.1.12, also known as GPx-1, Cellular glutathione peroxidase, Phospholipid-hydroperoxide glutathione peroxidase GPX1) is encoded by the GPX1 gene (Gene ID: 2876) in human. Glutathione peroxidase 1 (GPX1) is one of the most important enzymes in the cellular defense against oxidative stress. GPX1 is a selenoprotein, containing a selenocysteine residue at its active site, which is critical for its catalytic activity. The enzyme typically exists as a tetramer, each monomer consisting of about 200 amino acids. The structure allows for the reduction of hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols, respectively. The primary function of GPX1 is to protect the organism from oxidative damage by reducing lipid hydroperoxides as well as hydrogen peroxide using glutathione as the electron donor. This activity is crucial in the detoxification of peroxides generated during metabolism and in protecting biomembranes and other cellular components from oxidative stress. GPX1 is ubiquitously expressed in nearly all mammalian tissues, with particularly high levels in the liver and kidney. The expression of GPX1 can be induced by oxidative stress, and it is regulated by dietary selenium intake, as selenium is a cofactor for the enzyme. Altered expression of GPX1 has been linked to various cancers. Low GPX1 activity might contribute to the oxidative environment that is conducive to cancer development, whereas some cancer cells may upregulate GPX1 as a protective mechanism against oxidative stress. GPX1 deficiency has been associated with increased risk of cardiovascular diseases, including atherosclerosis and hypertension, likely due to the inability to adequately clear peroxides. Oxidative stress is a feature of many neurodegenerative diseases such as Alzheimer′s and Parkinson′s disease. GPX1, through its antioxidant function, is thought to play a protective role in the nervous system.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable