ZRB2824

Anti-SIRPA Antibody, clone 3N8 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, Tyrosine-protein phosphatase non-receptor type substrate 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3N8, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 55 kDa; observed mol wt ~60 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: C-terminal extracellular domain
• Protein ID accession no.: NP_001035111
• UniProt accession no.: P78324
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... SIRPA(140885)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3N8 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects SIRPA. It targets an epitope within 26 amino acids from the C-terminal extracellular domain. Might crossreact with SIRPB1-Isoform 3.

Immunogen

KLH-conjugated linear peptide corresponding to 26 amino acids from the C-terminal extracellular domain of human SIRPA.

Application

Quality Control Testing

Evaluated by Western Blotting in U-937 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SIRPA in U-937 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SIRPA in SK-MEL-28 and human PBMC lysates.

Affinity Binding Assay: A representative lot of this antibody bound SIRPA peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SIRPA in U-937 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected SIRPA in human cerebral cortex tissue sections.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected SIRPA in one million human PBMCs.


Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Tyrosine-protein phosphatase non-receptor type substrate 1 (UniProt: P78324; also known as SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs (Bit), CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1 (Sirp-alpha-1), Signal-regulatory protein alpha-2 (Sirp-alpha-2), Signal-regulatory protein alpha-3 (Sirp-alpha-3), p84) is encoded by the SIRPA (also known as BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP) gene (Gene ID: 140885) in human. Tyrosine-protein phosphatase non-receptor type substrate 1 (SIRPA) is a type I membrane protein and is characterized by its immunoglobulin-like domains located in the extracellular region, a single transmembrane segment, and a cytoplasmic region containing immunoreceptor tyrosine-based inhibition motifs (ITIMs). SIRPA is primarily involved in negative regulation of immune cell activation. It acts as a receptor for CD47, a signal-regulatory protein expressed on the surface of many cells. When engaged with CD47, SIRPA transmits a signal that inhibits phagocytosis by macrophages, thus playing a crucial role in self-recognition and protection of healthy cells from immune clearance. Additionally, the ITIM domains of SIRPA recruit and activate phosphatases such as SHP-1 and SHP-2, which then modulate downstream signaling pathways, including those involved in the inhibition of cellular activation and proliferation. SIRPA is widely expressed on cells in the immune system, including macrophages, dendritic cells, neurons, and other tissue cells. Alterations in SIRPA signaling are implicated in a variety of diseases. In cancer, cells often overexpress CD47 to engage SIRPA on immune cells, thereby evading phagocytosis and promoting tumor survival and growth. Furthermore, dysregulation of SIRPA has been associated with autoimmune diseases, where impaired signaling may contribute to the destruction of healthy cells.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable