ZRB2310
Anti-PKCγ Antibody, clone 2B9 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Protein kinase C gamma type;EC:2.7.11.13;PKC-gamma
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2B9, recombinant monoclonal
description
2B9 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 78.45 kDa
observed mol wt ~78 kDa
purified by
using Protein A
species reactivity
rat, human
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
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sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... PRKCG(5582)
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2B9 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects PKC . It targets an epitope within 21 amino acids from the C-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human PKC .
Application
Quality Control Testing
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKC in Rat brain tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PKC in Human brain and Rat brain tissue lysates.
Affinity Binding Assay: A representative lot of this antibody bound PKC with a KD of 1.4 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PKC in human cerebellum tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected PKC in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKC in Rat brain tissue lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PKC in Human brain and Rat brain tissue lysates.
Affinity Binding Assay: A representative lot of this antibody bound PKC with a KD of 1.4 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected PKC in human cerebellum tissue sections.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected PKC in human cerebellum tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Protein kinase C gamma type (UniProt: P05129; also known as EC:2.7.11.13, PKC-gamma) is encoded by the PRKCG (also known as PKCG) gene (Gene ID: 5582) in human. Protein kinase C (PKC), a ubiquitous, phospholipid dependent serine/threonine kinase that is involved in signal transduction associated with cell proliferation, differentiation, and apoptosis. At least eleven closely related PKC isozymes have been reported that differ in their structure, biochemical properties, tissue distribution, subcellular localization, and substrate specificity. PKCγ is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine kinase that plays diverse roles in neuronal cells and eye tissues. It binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. The catalytic domain of PKCγ contains an ATP-binding site. Its regulatory domain has a Ca2+ binding site. The amino terminal half of the regulatory domain contains two conserved regions, C1 and C2 that play a vital role in the regulation of enzyme activity. The catalytic domain is composed of highly conserved C3 and C4 regions. The C3 region contains the ATP-binding consensus sequence, whereas the C4 region is responsible for protein substrate binding. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Saito, N., and Shirai, Y. (2002). J. Biochem. 132(5); 683-687; Coussens, L., et al. (1986). Science. 233(4766); 859-866).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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