U0880
Uricase from Candida sp.
recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid
Synonym(s):
Urate: oxygen oxoreductase
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About This Item
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recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
≥2 units/mg solid
mol wt
35 kDa by SDS-PAGE
storage temp.
−20°C
InChI
1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
InChI key
VPXDHZMLJOJKOX-UHFFFAOYSA-N
General description
Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.
Application
Uricase from Candida sp. has been used:
- to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
- to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
- for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors
Biochem/physiol Actions
Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.
Unit Definition
One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.
Physical form
Powder; contains citrate and sucrose
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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