T2011

Trypsin inhibitor from chicken egg white

Type III-O (free of ovoinhibitor)

• Synonym(s): Ovomucoid
• CAS Number: 9035-81-8
• EC Number: 232-906-9
• MDL number: MFCD00082102
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• biological source: chicken egg white
• Quality Level: 300
• type: Type III-O (free of ovoinhibitor)
• form: powder
• mol wt: 14 kDa
• solubility: water: soluble 10 g/L
• storage temp.: 2-8°C

Description

General description

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Application

Trypsin inhibitor from chicken egg white has been used:

• to terminate proteolysis reaction
• in OVO-1 solution to triturate the cell suspension for the dissociation of retina
• in phosphate buffered saline for dissociation of cortices

Biochem/physiol Actions

Ovomucoid possesses trypsin inhibitor activity. It has several conformational and linear epitopes that can be bound by immunoglobulin E (IgE). It is highly stable against heat and proteolysis.

Unit Definition

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Other Notes

View more information on Trypsin Inhibitor.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317 - H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves