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Key Documents

SAB4700450

Sigma-Aldrich

Monoclonal Anti-CD56-APC antibody produced in mouse

clone MEM-188, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-NCAM, Anti-NCAM1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

conjugate

Allophycocyanin conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-188, monoclonal

form

buffered aqueous solution

species reactivity

nonhuman primates, human

technique(s)

flow cytometry: suitable

isotype

IgG2a

NCBI accession no.

NM_000615.5

UniProt accession no.

P13591

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... NCAM1(4684)

General description

The antibody MEM-188 reacts with a 180 kDa isoform of CD56 (NCAM) expressed in leukocytes. It has been suggested that the antibody MEM-188 could react with rhesus monkey lymphocytes. Reactivity with other NCAM isoforms has not been tested.

Immunogen

KG-1 human acute myelogenous leukemia cell line

Application

The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Carrie E Gifford et al.
Cytometry. Part B, Clinical cytometry, 86(4), 263-271 (2014-03-13)
X-linked lymphoproliferative disease is caused by mutations in two genes, SH2D1A and XIAP/BIRC4. Flow cytometric methods have been developed to detect the gene products, SAP and XIAP. However, there is no literature describing the accuracy of flow cytometric screening performed
K Drbal et al.
Blood, 98(5), 1561-1566 (2001-08-25)
An unusual CD18 monoclonal antibody (mAb) MEM-148 binds, in contrast to standard CD18 mAbs, specifically to peripheral blood monocytes and neutrophils activated by various stimuli such as phorbol myristate acetate, opsonized zymosan, heat-aggregated immunoglobulin, and (after priming with lipopolysaccharide, tumor

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