SAB1306082
ANTI-HIS TAG antibody produced in rabbit
purified immunoglobulin, buffered aqueous solution
Synonym(s):
Rabbit anti-hexa histidine tag
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
rabbit
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
western blot: 1:1000
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Histidine has a strong association with immobilized metal ion matrices. This is because the imidazole ring has electron donor groups that form coordination bonds with the transition metal that is immobilized. Hexahistidine tag (His-tag) consists of four to six histidines that are fused genetically. It is mainly used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). His-tags are added at the amino or carboxy termini of proteins.
Physical form
Supplied in PBS with 0.09% (W/V) sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Tandem Immobilized Metal-Ion Affinity Chromatography/Immunoaffinity Purification of His-tagged Proteins? Evaluation of Two Anti-His-Tag Monoclonal Antibodies
Kristian M.Muller, et al.
Analytical Biochemistry, 54-61 (1998)
[16] Purification of Proteins Using Polyhistidine Affinity Tags
Joshua A, et al.
Methods in Enzymology (2000)
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