R8526

RO-52 (SSA) human

recombinant, expressed in E. coli, liquid

• MDL number: MFCD03458409
• UNSPSC Code: 12352202
• NACRES: NA.56

Properties

• recombinant: expressed in E. coli
• Quality Level: 200
• form: liquid
• mol wt: 52 kDa by SDS-PAGE
• technique(s): ELISA: suitable
• UniProt accession no.: P19474
• storage temp.: −70°C
• Gene Information: human ... TRIM21(6737)

Description

General description

RO-52 (SSA), also called as tripartite motif-containing protein 21 (TRIM21) is a 52 kDa, ubiquitin E3 ligase. It has a N-terminal RING finger domain (Really Interesting New Gene), crucial for ubiquitination functionality. RO-52 (SSA) gene is mapped to human chromosome 11p5.5. It is capable of autoubiquitination and uniquitylates p62 protein and regulates redox homeostasis. Patients with Sjogren′s syndrome display elevated levels of anti-Ro/SSA antibodies. RO-52 levels in peripheral blood mononuclear cells (PBMC) contributes to pathogenesis of systemic lupus erythematosus (SLE), systemic autoimmune rheumatic diseases (SARDs), interstitial lung disease (ILD) and connective tissue diseases (CTD). RO-52 is considered as potential target for treating SLE.

Application

RO-52 (SSA) human has been used:

• as a component of the autoantigen array
• to immobilise protein on the self-assembly monolayer
• in enzyme-linked immunosorbent assays (ELISAs)

Biochem/physiol Actions

Used for the detection and quantification of antibodies to RNPs in rheumatic diseases.

Physical form

solution contains 20 mM phosphate buffer, 500 mM NaCl, 2M - 3M urea, pH 6.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable