Custom qPCR Probes

Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. TAMRA is not a dark quencher and therefore contributes to an overall increase in background because of its own native fluorescence. DABCYL, though a dark quencher, has an inadequate absorption footprint that overlaps very poorly with reporter dyes emitting above 480 nm.

Molecular Beacons are structured probes that are highly sensitive, sequence specific, and are used for sequence detection in qPCR and in vitro studies.

Hudnall, et al. demonstrates the use of easy and effective qPCR assys to screen blood for herpesvirus.

Onxy Quencher emits heat instead of light and offers and improved signal-to-noise ratio over fluorescent quenchers.

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.

For more complex and demanding applications, utilize our consultative service to ensure the success of your assay.

We offer OligoEvaluator™ as an easy-to-use Oligo Analyzer tool for online oligonucleotide sequence calculator that provides primer dimer analysis values for PCR and calculation of T_m.

Custom oligo Blanket Purchase Order tracking system simplifies expenditure management for customers.