PROBES
Custom qPCR Probes
Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR
Synonym(s):
BHQ, BHQ Probes, Black Hole Quencher, Locked Nucleic Acid probes, Probes, Real-Time PCR Probes, locked nucleic acid, qPCR assays, qPCR designs
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Articles
Hudnall, et al. demonstrates the use of easy and effective qPCR assys to screen blood for herpesvirus.
Onxy Quencher emits heat instead of light and offers and improved signal-to-noise ratio over fluorescent quenchers.
Dual-Labeled Probes in Digital PCR
Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR. TAMRA is not a dark quencher and therefore contributes to an overall increase in background because of its own native fluorescence. DABCYL, though a dark quencher, has an inadequate absorption footprint that overlaps very poorly with reporter dyes emitting above 480 nm.
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LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.
We offer OligoEvaluator™ as an easy-to-use Oligo Analyzer tool for online oligonucleotide sequence calculator that provides primer dimer analysis values for PCR and calculation of Tm.
Key components of our QMS (quality management system) include: certificates of analysis; document control; change notification; vendor management; business agreements; and, corrective and preventive actions.
Label licenses for our oligonucleotide products, including Black Hole Quencher®, BlackBerry® Quencher, and Locked Nucleic Acid (LNA®).
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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